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Sibande et al

Journal of Dynamics in Agricultural Research

Available online at

Vol.2(5), pp.46-53, December  2015

Article ID: jdar/15/020

Copyright © 2015


Original Research Paper

Effect of priming techniques and seed storage period on soybean (Glycine max l) germination

G.A.K. Sibande*, V.H. Kabambe, M.F.A Maliro, V. Karoshi

Department of Crop and Soil Sciences, Lilongwe University of Agriculture and Natural Resources (LUANAR), Bunda College Campus, P.O Box 219, Lilongwe, Malawi.

*Corresponding author. E-mail:


Received 7 September, 2015; Accepted 9 November, 2015.


Inadequate supply of improved seed varieties is one of the major constraints to soybean production in Malawi. Consequently, most of the seeds being planted by small scale farmers were obtained from open grain markets or derived from previous harvest some of which were already overage, exposed to poor storage conditions and likely suffered certain degree of deterioration. Use of such aged soybeans for planting, predisposes the seeds to imbibitional injury due to their hygroscopic nature thereby resulting in poor germination. Seed quality could be improved by pre-treatment techniques such as seed priming. An experiment was designed to study the effect of seed priming using water, potassium nitrate (KNO3), Moringa oleifera leaf powder (MLP), custard apple (Annona reticulata L) leaf powder (CALP) and a control based on the rate of germination, germination percentage and other seedling vigour traits in three soybean seed lots of Serenade with different storage durations in June, 2014. The trial was conducted in the laboratory in a completely randomized design (CRD) with four replicates to determine the effect of seed priming and storage period on germination traits, root and shoot lengths, seedling dry weight and seed vigour index on Glycine max L. Seed priming methods had significant (P<0.001) effect on soybean germination parameters. However, results showed a significant reduction in rate of germination and germination percentage as a result of seed priming. The unprimed (control) soybean seed had the highest germination results. There was significant reduction in germination percentage with increase in storage period. The highest rate of germination and germination percentage were recorded in the freshly harvested seed (control) while the lowest rates were attained in two years old seed. The results therefore showed that soybean seed priming may not always lead to beneficial effect on the rate of germination, germination percentage and other seedling vigour traits but seed storage duration.

Key words: Glycine max L., seed priming, seed storage, germination percentage, germination rate.