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Iliyasu et al.



Dynamic Journal of Pure and Applied Microbiology

Available online at http://www.journaldynamics.org/djpam

Vol.1(1), pp.1-9, July 2016

Article ID: DJPAM/JDYA75013619

Copyright © 2016

 

Original Research Paper

Urovirulence phenotypes and multidrug resistant pattern of Escherichia coli isolates from clinical samples

Iliyasu, M.Y*, Uba, A., and Agbo, E.B.

Biological Sciences Department, Abubakar Tafawa Balewa University, ATBU, Bauchi, Nigeria.

*Corresponding Author. E-mail:mahmudyerimailiyasu@yahoo.com.

Received 23 April, 2016; Accepted 24 June, 2016.

Abstract

This study was carried out to determine phenotypic characteristics of some of the virulence factors and multidrug resistant pattern associated with uropathogenic Escherichia coli (UPEC) isolates from some health centres in Bauchi metropolis, Nigeria.  A total of 56 isolates were recovered from various UTIs cases with 7 from control subjects. The age of the patients ranges from 1 to 70 years and above. Highest number of isolates (37.5%) was in UTI cases within age group of 31 to 40 years, followed by (19.6%) in 21 to 30 years of age; mostly in females (66.1%) out-patients (75.0%). An average of 35 (62.5%) of the E. coli isolates were resistant to 3 or more antimicrobial agents (Multidrug resistant). High resistance was observed in Ampicillin (87.5%), Amoxycillin (85.2%), the newer generation Cephalosporins like Ceftazidime (66.1%) and Cefuroxime (67.9%). The isolates were appreciably sensitive to Ceftriazone (62.5%), Amikacin (75.0%) and Imipenem (83.9%). Fimbrial adhesins is the most frequent virulence factor (76.5%) in the in-patients, 74.3% in the female patients. This is followed by haemolysin 66.7% (out-patients) and 69.3% (male).  Multiple virulence phenotypes were produced with prevalence among 41.0% of the out-patients and 33.3% of the male. UPEC were frequently isolated from cases of bacteriuria in female (31.4%), followed by pylonephritis (22.9%) in the same group. Pylonephritis, urosepsis and urethritis was the predominant (19.0%) cases observed among the male. Multidrug resistance is on the rise among UPEC isolates, therefore knowledge of the resistance patterns of UPEC isolates will help to guide appropriate and judicious use of antimicrobial agents.

Keywords: Uropathogenic E. coli (UPEC), Virulence factors, Multidrug resistance, Fimbrial adhesins, Haemolysin, Bacteriuria, Phylonephritis and Urethritis.